Journal: Heliyon
Article Title: Unveiling a cuproptosis-related risk model and the role of FARSB in hepatocellular carcinoma
doi: 10.1016/j.heliyon.2024.e32289
Figure Lengend Snippet: FARSB knockdown affects HCC cell growth and cell cycle. (A) RT-PCR analysis of FARSB expression in various HCC and normal liver cells. Confirmation of efficient FARSB knockdown in Hep-G2 (B) and Huh7 (C) cells. Cell viability reduction in FARSB knockdown HepG2 (D) and Huh7 (E) cells via CCK-8 assay. (F, G) Clone formation assay for assessing cell proliferation ability in Huh7 and Hep-G2 cells. (H) Flow cytometry shows cell cycle arrest at G1 phase after FARSB knockdown.
Article Snippet: The HCC-derived cell lines HepG2, Huh7, and HCCLM3, as well as the normal liver cell line L02, were procured from the American Tissue Culture Collection (ATCC) and employed for in vitro experimentation.
Techniques: Knockdown, Reverse Transcription Polymerase Chain Reaction, Expressing, CCK-8 Assay, Tube Formation Assay, Flow Cytometry